MEG Laboratories

 

Phylogenetic analysis computer

The labs of the MEG at the ISE-Verbania are equipped for most of the analyses required in microbial ecology. Spaces are divided in (i) microscopy and single cell analysis lab, (ii) PCR lab, (iii) radioactivity lab, (iv) RT-PCR lab, (v) limnological analysis lab, and two experimental culture rooms. MEG facilities include epifluorescence and inverted microscopes (Zeiss), stereomicroscopes (Olympus), an automated homogenizer (Precellys), PCR and RT-PCR machines and related tools (Bio-Rad), a flow-cytometer (BD), an automated spectrofluorimetric plate reader (Promega), carbon and phosporous measuring instruments (Shimadzu), a scintillation counter (Beckmann), a multiparametric pelagic probe (Idronaut Ocean Seven), phyto-pam (Walz), a 12-vessels chemostat (VT Italy, Watson-Marlow), six benches (for radioactivity meaurements, chemicals, cultivation, PCR and RT-PCR), two climatized rooms, freezer and refrigerators (including a -80°C), and a number of other devices including DGGE facilities (Bio-Rad), micro and mesocosms, and a DNA sequencer (Applied Biosystems).

A dedicated ARB-Qiime-Mothur high-performance computer is available for advanced phylogenetical analysis.

The shared instrumentation at the ISE includes a dedicated boat on Lake Maggiore, equipped for offshore sampling and in situ measurements, two large artificial experimental ponds, sampling boats, cars and a minivan.

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